Can duplicate pg_hba entries result in a connection error?

I have received the following error when attempting to connect to an Arch Linux server:

Sequel::DatabaseConnectionErrorreplication#monitor
PG::Error: could not connect to server: Connection refused Is the server running on host "45.56.86.24" and accepting TCP/IP connections on port 5432?

but I have an entry in my pg_hba.conf file on that server:

hostssl replication replication 45.56.86.24/32 md5

Actually... there are two lines like this. Is presence of the second line be expected to result in this error?